HOUSTON –Nov. 28, 2018 – To help patients with muscle disorders, scientists at The University of Texas Health Science Center at Houston (UTHealth) have engineered a new stem cell line to study the conversion of stem cells into muscle.
Muscle disorders such as muscular dystrophy cause muscles to weaken and deteriorate, and they affect more than 50,000 people in the United States. Symptoms include difficulty walking and standing. In severe cases, the disorders might involve cardiac and respiratory muscles and lead to death. There is no cure.
Darabi’s team engineered a novel human stem cell line for skeletal muscle. To ensure the purity of the muscle stem cells, they tagged muscle genes (PAX7, MYF5) with two fluorescent proteins. “In order to improve the formation of the muscle from stem cells, we screened several bioactive compounds. We were also able to observe muscle stem cell activity in great detail using color tags,” he said.
#CRISPR/Cas9 #Molecular Medicine #AX7, MYF5